How to optimize the ACQUITY QDa Mass Detector SIR conditions? - WKB82044
Article number: 82044
OBJECTIVE or GOAL
Optimize the ACQUITY QDa Mass Detector SIR conditions
ENVIRONMENT
- ACQUITY QDa Mass Detector
PROCEDURE
- Create an instrument method with about 6 lines of MS Scan function added
- Set the Start Mass/End Mass specified in each function within the range including m/z of the target component.
- Set different cone voltages for each function (10-25V for Positive, 20-35V for Negative)
- When setting the number of functions and the range of the Start Mass/End Mass, be careful so that the actual Sampling Rate won’t be too insufficient.
- Specify the instrument method created in step 1 and measure the standard.
- If you want to shorten the time, connect a longer PEEK tube instead of the column, and adjust to make the wide peaks elute immediately after injection.
- From the scan data acquired in step 2, confirm the target component m/z and the optimum value for Cone Voltage.
- Create a new instrument method with SIR functions added, enter the m/z and Cone Voltage obtained in step 3.
- Connect the column, specify the instrument method created in step 4 (LC conditions were changed for column measurement), and perform standard measurement.
- Check the number of points within the elution time of the peak, if insufficient, increase the sampling rate (at least 15 points per peak)
- Check the measured data from step 5, if the sensitivity is insufficient, create multiple instrument methods with different Capillary Voltage and measure the standard several times
(Set to 0.3-1.5 kV for positive and 0.3-0.8 kV for negative) - Confirm the measured data from step 6 and enter the optimum capillary voltage value into the SIR instrument method
- Set the Probe Temperature using the following as a goal.
Flow rate 0.3 mL/min or more: Use the initial value of 600 °C
Flow rate 0.25-0.3 mL/min: Use 300 °C