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How to clean the ACQUITY UPLC Classic, H-Class and I-Class Systems? - WKB82038

Article number: 82038

OBJECTIVE or GOAL

Clean the ACQUITY UPLC H-Class and I-Class Systems

ENVIRONMENT

  • ACQUITY UPLC (Classic)
  • ACQUITY UPLC H-Class
  • ACQUITY UPLC H-Class Bio
  • ACQUITY UPLC H-Class PLUS
  • ACQUITY UPLC H-Class PLUS Bio
  • ACQUITY UPLC I-Class
  • ACQUITY UPLC I-Class PLUS

PROCEDURE

  1. Prepare the washing solvent
  2. Prepare the washing solvent in a sample vial
  3. Remove columns
  4. Remove the solvent filter
  5. Prime all lines except seal washing line with the washing solvent
  6. Create the Instrument Method used for cleaning
    A/B/C/D=25/25/25/25 Flow rate: 0.2mL/min
  7. Create the Sample Set used for cleaning
    Injection Volume: Needle (including the expansion loop) volume
    Run Time: 1 minute
    Number of Injections: 50 to 100
  8. Rinse all the lines with Methanol before going back to the original solvents used in the method.

ADDITIONAL INFORMATION

For the washing solvent, select one which can dissolve the samples analyzed routinely.
If you can't find the appropriate solvent, use 1:1:1:1 water / acetinitrile / methanol / isopropanol with 0.2% formic acid.
If alkaline solution is expected to be more effective, 0.2% formic acid can be replaced with 0.2% aqueous ammonia.
In either case, after cleaning, replace the washing solvent with water and perform the above cleaning procedures to switch to water thoroughly.
Even after completing 30 injections, it is more effective to keep the washing solution running if possible.
To give a rough idea, prepare 1L of washing solvent and use it all.
If you want to shorten the time, perform the cleaning at a higher flow rate.

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