2996 filter initialization failure with new flow cell - WKB75985

SYMPTOMS

• Filter error occurs upon initialization
• Diagnostics result in Shutter = BAD: shutter fails, light spectrum bad, average 2650, error code 0
• New flow cell

ENVIRONMENT

• 2996 PDA Detector

CAUSE

Flow cell was not installed properly

FIX or WORKAROUND

Note: You do not need to shut down the 2996 Detector to remove and replace the flow cell. If the lamp is on, it may not be a bad idea to turn the detector off.

1. Required materials:
   • 5/16-inch open-end wrench
   • Phillips screwdriver
   • Powder-free gloves 
2. To remove the 2996 Detector flow cell:
   1. Set the flow to 0.0 mL/min.
   2. Power-off the solvent delivery system or pump to avoid exposure to chemicals.
   3. Use the 5/16-inch wrench to disconnect the fluidics lines at the front of the detector.

      • Caution: To avoid the possibility of leaking mobile phase, do not disconnect the inlet or outlet fluidics lines while there is pressure in the chromatographic system. Always vent your system before disconnecting fluidics lines.

   4. Lift up the 2996 detector front cover and pull the front cover from the detector chassis.
   5. Open the flow cell access door by pulling the black thumb tab, and then pull the door gently toward you.
   6. Use the Phillips screwdriver to loosen the three thumbscrews that hold the flow cell assembly to the optics bench and the thumbscrew that secures the bracket holding the fluidics connections, and then detach the bracket.
   7. Pull the flow cell assembly gently toward you to remove it from the detector.
3. To install the 2996 Detector flow cell assembly:
   1. Attention: The alignment of the flow cell in the optics bench is critical to detector operation. Be careful not to damage the flow cell body.
   2. While you hold the flow cell assembly in a vertical orientation, insert the assembly into the optics bench.
      • Note that the flow cell is self-aligning and uses the guide pins on the optics bench.
   3. Gently push the front of the assembly until it seats on the front alignment pins.
   4. Hand-tighten the thumbscrews. It may be easier to start the top thumbscrew first, then the bottom screws.
      • Attention: Be careful not to overtighten the screws.
   5. Reconnect the fluidics lines.

ADDITIONAL INFORMATION

Refer to the 2996 PDA Detector Operator's Guide, Sections 3.1.3 and 3.1.4 of Revision C for additional information.