Alliance 2695 with 2487 high pressure with column - WKB70001

SYMPTOMS

• High pressure during flow (around 4,000 psi)
• Disconnecting the column outlet tubing causes the pressure to drop

ENVIRONMENT

• Alliance HPLC System
• 2695 Separations Module
• 2487 Dual-Wavelength Absorbance Detector
• Use of phosphate buffer on system

CAUSE

Clog in flow cell

FIX or WORKAROUND

1. Flush flow cell.
2. Follow the Cleaning Procedure on Page 20, Table 1, cleaning condition 3 in Controlling Contamination in LC/MS Systems - Best Practices (715001307)

ADDITIONAL INFORMATION