How to calibrate an ACQUITY UPLC FLR Detector - WKB56294

OBJECTIVE or GOAL

Follow the protocol for calibrating the ACQUITY UPLC FLR module.

Two parameters must be calibrated within this optical detector before using the instrument:

• The fluorescence wavelength (Excitation and Emission)
• The PMT (PhotoMultiplier Tube) sensitivity response

ENVIRONMENT

• ACQUITY UPLC FLR Detector

PROCEDURE

Note that the lamp must be lit for at least an hour before starting the calibration processes.

To calibrate the ACQUITY UPLC FLR, run 100% water through the system. [Ensure that the water is clean. Request MS-grade solvent when working with mass spectrometers.]
In Service or Maintenance mode, perform the following setup in the exact order shown:
- Calibrate wavelength (approx. 2 minutes)
- PMT Calibration (approx. 2 minutes; a pass is for R > or = 0.999)
- Normalize units (approx. a few seconds). RAMAN gain would be about 200 for new modules or modules with a new lamp fitted.

See the following useful document link: Getting Started Guide

ADDITIONAL INFORMATION

In a UNIFI environment, if a request to select a calibration curve appears after completion of calibration procedures but before a successful run of the FLR precision test (see screenshot),

click "Cancel" and then "Forward". The request clears.