What is the best way to remove air from the solvent lines of a 1525 pump after running out of mobile phase? - WKB4938

ENVIRONMENT

• 515 HPLC Pump
• 1515 Isocratic HPLC Pump
• 1525 Binary HPLC Pump
• 1525 Micro Binary HPLC Pump

ANSWER

Manually prime using a priming syringe: Insert the priming syringe into the Luer fitting at the center of the draw-off valve handle. Turn the handle counterclockwise, about 1/2-turn, to open the valve, and pull back on the plunger to remove air bubbles. Once the air is removed, close the valve and start flow. 

Flush the system with 100% methanol for a minimum of 30 minutes. Note: If methanol is not miscible with your mobile phase, use an intermediate mobile phase.

ADDITIONAL INFORMATION