Poor reproducibility on H-Class - WKB48003
Article number: 48003
SYMPTOMS
- Intermittently poor reproducibility (% RSD) based on peak area and height for an established customer method
- All system diagnostics pass
- System SQT precision tests pass
ENVIRONMENT
- Empower
- ACQUITY UPLC H-Class
CAUSE
The use of methanol as sample diluent causes high variability with default sample manager settings.
FIX or WORKAROUND
Reduce the sample draw rate to 15 µL/min and add a 0.2-µL post-draw air gap.
ADDITIONAL INFORMATION
id48003, EMP2LIC, EMP2OPT, EMP2SW, EMP3GC, EMP3LIC, EMP3OPT, EMP3SW, EMPGC, EMPGPC, EMPLIC, EMPOWER2, EMPOWER3, EMPSW, SUP, UPQSM, UPQSM+, UPQSMBIO, UPQSMBIO+, UPSMFTN, UPSMFTN+, UPSMFTNB+, UPSMFTNBIO