Poor reproducibility on H-Class - WKB48003
Article number: 48003
SYMPTOMS
- Intermittently poor reproducibility (% RSD) based on peak area and height for an established customer method
- All system diagnostics pass
- System SQT precision tests pass
ENVIRONMENT
- Empower
- ACQUITY UPLC H-Class
CAUSE
The use of methanol as sample diluent causes high variability with default sample manager settings.
FIX or WORKAROUND
Reduce the sample draw rate to 15 µL/min and add a 0.2-µL post-draw air gap.
ADDITIONAL INFORMATION