Inconsistent injection volumes and poor reproducibility after a new needle was installed on 2695.
- Alliance HPLC System
- 2695 Separations Module
Purge was done with 100% water only after needle replacement.
FIX or WORKAROUND
- Thoroughly purge injector 20 times with 50/50 Methanol/Water, and then finally with 100% Methanol .
- Loosen the syringe screw at the bottom and manually purge to remove air bubbles.