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Poor reproducibility after replacing 2695 sample needle - WKB397

Article number: 397

SYMPTOMS

Inconsistent injection volumes and poor reproducibility after a new needle was installed on 2695.

ENVIRONMENT

  • Alliance HPLC System
  • 2695 Separations Module

CAUSE

Purge was done with 100% water only after needle replacement.

FIX or WORKAROUND

  1. Thoroughly purge injector 20 times with 50/50 Methanol/Water, and then finally with 100% Methanol .
  2. Loosen the syringe screw at the bottom and manually purge to remove air bubbles.

ADDITIONAL INFORMATION

 

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