- Failed to calibrate system volume message when running needle and loop volume characterization.
- ACQUITY UPLC Sample Manager (SM)
Slight bend in the needle.
FIX or WORKAROUND
- Reinstall needle after straightening slight bend.
- Perform the z axis characterization, needle seal characterization, and needle/loop volume characterization.
There are detailed instructions in the user's guide on needle replacement and the characterizations that must be completed after needle reinstallation - ACQUITY UPLC System Operator's Guide.