How to replace an Active Preheater (APH) - WKB320480
OBJECTIVE or GOAL
Replace an Active Preheater (APH)
ENVIRONMENT
- ACQUITY UPLC H-Class
- ACQUITY UPLC H-Class Bio
- ACQUITY UPLC I-Class
- ACQUITY UPLC H-Class PLUS
- ACQUITY UPLC H-Class PLUS Bio
- ACQUITY UPLC I-Class PLUS
- ACQUITY UPLC Column Manager
- ACQUITY UPC2
- ACQUITY Premier System
PROCEDURE
Replacing the active preheater assembly
To prevent burn injuries, set the column temperature to Off and then allow the column compartment and its components to cool for 60 minutes before touching them. Monitor the column compartment’s internal temperature to ensure all components are cool.
To prevent contaminating system components, wear clean, chemical-resistant, powder-free gloves when replacing the active preheater assembly.
To avoid damaging the active preheater assembly, turn the column, not the fitting, when installing or removing the column to prevent the active preheater tubing from turning with the fitting.
To remove the active preheater assembly:
1. Stop any running mobile phase before replacing the active preheater assembly.
2. After allowing sufficient time for the instrument to cool, open the column heater, column manager, or auxiliary column manager door.
3. Disconnect the inlet tubing:
• Column heater – disconnect the tubing from injector port 6 of the sample manager.
• 30-cm column heater – disconnect the tubing from injector port 6 of the sample manager injector port of the switching-valve.
The port varies depending on which active preheater you are replacing.
• Auxiliary column manager – disconnect the tubing from the appropriate injector port of the switching-valve, located in the column manager.
4. Follow these steps for a column manager or auxiliary column manager:
a. Push the latches on top of the column compartment cover inward, to disengage them from the latch pins.
b. Tilt the column compartment cover forward just enough to clear the valve drip tray.
c. Pull the column compartment cover up and away from the column manager or auxiliary column manager.
4. While holding the column, disconnect the column outlet tubing.
5. While holding the gold active preheater fitting, rotate the column off the fitting.
To install the new active preheater assembly:
1. Loosen the stainless steel cap nut from the gold fitting.
2. Finger tighten the gold fitting, with the ferrule, into the column.
3. Holding the active preheater’s dual-threaded fitting in place, rotate the column or optional in-line filter onto the fitting until it is snug, and then tighten an additional 3/8-turn.
5. Carefully route the active preheater assembly inlet tubing to the valve:
• Column heater – route the tubing through the groove on the side of the column compartment, and then through the cutout in the top of the sample manager door to the injector. Do not route the tubing behind the sample manager door hinge.
• 30-cm column heater – route the tubing through the column heater door.
Warning: Never connect the active preheater in the upper and lower positions at the same time, doing so can damage the 30-cm columnheater.
• Auxiliary column manager – route the tubing between the column compartment and the side of the instrument chassis to the switching valve.
6. Remove the O-ring retainer from the inlet end of the active preheater assembly tubing, and then connect the inlet tubing to the valve:
• Column heater – connect the tubing to injector port 6 of the sample manager.
• 30-cm column heater – connect the tubing to injector port 6 of the sample manager.
• Column manager – connect the tubing to the appropriate injector port of the switching-valve. The port varies depending on whichactive preheater you are replacing.
• Auxiliary column manager – connect the tubing to the appropriate injector port of the switching valve, located in the column manager.
The port varies depending on which active preheater you are replacing.
ADDITIONAL INFORMATION
- H-Class APH = 205000730
- H-Class Bio APH = 205001754
- H-Class APH SS = 205001774
- H-Class APH SS 18.5LG = 205000774
- I-Class APH = 205000894
- I-Class Plus APH = 205001757
- UPC2APH = 205001002
- ACQUITY Premier = 205002234
For a complete list of Active Preheaters, see articleWKB7232.
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