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Waters

Peak area not reproducible with ACQUITY Arc- WKB26800

Article number: 26800

SYMPTOMS

  • Area count highly variable
  • Peak integration is fine
  • Priming all lines doesn't fix the issue
  • Retention time is reproducible

ENVIRONMENT

  • ACQUITY Arc
  • ACQUITY UPLC H-Class Bio
  • ACQUITY UPLC H-Class

CAUSE

Worn out sample syringe

FIX or WORKAROUND

  1. Replace the sample syringe.
  2. Confirm that the area counts are reproducible.

ADDITIONAL INFORMATION

Air in the sample syringe is a common cause of incorrect area count. Perform at least 40 cycles of prime before starting an injection.

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