- Area count highly variable
- Peak integration is fine
- Priming all lines doesn't fix the issue
- Retention time is reproducible
- ACQUITY Arc
Worn out sample syringe
FIX or WORKAROUND
- Replace the sample syringe.
- Confirm that the area counts are reproducible.
Air in the sample syringe is a common cause of incorrect area count. Perform at least 40 cycles of prime before starting an injection.