Peak area not reproducible with ACQUITY Arc- WKB26800
Article number: 26800
SYMPTOMS
- Area count highly variable
- Peak integration is fine
- Priming all lines doesn't fix the issue
- Retention time is reproducible
ENVIRONMENT
- ACQUITY Arc
- ACQUITY UPLC H-Class Bio
- ACQUITY UPLC H-Class
CAUSE
Worn out sample syringe
FIX or WORKAROUND
- Replace the sample syringe.
- Confirm that the area counts are reproducible.
ADDITIONAL INFORMATION
Air in the sample syringe is a common cause of incorrect area count. Perform at least 40 cycles of prime before starting an injection.
id26800, UPAQSM, UPARBQSM, UPARBSMFTN, UPARCH30, UPARHQSM, UPARQSM, UPARSMFTN, UPPDAARCB