SM-FTN system overpressure caused by blockage in Vespel seat or seat port tubing and/or needle - WKB257160

SYMPTOMS

• BSM/QSM: Overpressure error
• Overpressure caused by blockage in Vespel seat or seat port tubing and/or needle
• Sample fluidics high pressure limit

ENVIRONMENT

• ACQUITY UPLC H-Class - FTN
• ACQUITY UPLC I-Class Sample Manager - FTN
• ACQUITY Arc SM-FTN-R

CAUSE

Blockage in Vespel seat or seat port tubing and/or needle

FIX or WORKAROUND

1. Disconnect the needle or extension loop from the injection valve port 4 and the seat port tubing from injection valve port 1, and then connect those two fittings swapped.
2. Using a suitable mobile phase, considering solubility if you suspect precipitate, start to flush the system at 0.1 mL/min. Increase the flow (up to 0.5 mL/min) if the system does not go into overpressure.
3. Look for a significant drop in pressure, which could indicate that the blockage has cleared.
4. Continue to backflush the Sample Manager-FTN fluidics for at least 15 minutes.
5. Stop the flow.
6. Reconnect the needle or extension loop to the injection valve port 4 and the seat port tubing to injection valve port 1.
7. Set up the flow rate used in the work method and confirm that the pressure returns to normal values before starting the analysis.

ADDITIONAL INFORMATION