System overpressure error WKB24577
Article number: 24577
SYMPTOMS
- Overpressure error on H-Class without column
- The error disappears when disconnecting the column inlet tube from the flow cell
ENVIRONMENT
- ACQUITY UPLC H-Class
- ACQUITY UPLC I-Class
- ACQUITY UPLC
- UPLC TUV Detector
- UPLC PDA Detector
- Alliance HPLC System
- 2695 Separations Module
- 2695D Dissolution Separations Module
- 2487 Dual-Wavelength Absorbance Detector
- 2489 UV/Vis Detector
- 2996 PDA Detector
- 2998 Photodiode Array Detector
CAUSE
The flow cell is clogged.
FIX or WORKAROUND
- Purge the system with water.
- Replace the column with a union.
- Set the column temperature to 50 ºC.
- Reverse the flow cell inlet and outlet tubes.
- Start the flow at a very low rate, ensuring that the back pressure remains below the maximum rating of 1000 psi for the UV flow cell.
- Flush the flow cell until the clog is removed.