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Waters

System overpressure error WKB24577

Article number: 24577

SYMPTOMS

  • Overpressure error on H-Class without column
  • The error disappears when disconnecting the column inlet tube from the flow cell

ENVIRONMENT

  • ACQUITY UPLC H-Class
  • ACQUITY UPLC I-Class
  • ACQUITY UPLC
  • UPLC TUV Detector
  • UPLC PDA Detector
  • Alliance HPLC System
  • 2695 Separations Module
  • 2695D Dissolution Separations Module
  • 2487 Dual-Wavelength Absorbance Detector
  • 2489 UV/Vis Detector
  • 2996 PDA Detector
  • 2998 Photodiode Array Detector

CAUSE

The flow cell is clogged.

FIX or WORKAROUND

  1. Purge the system with water.
  2. Replace the column with a union.
  3. Set the column temperature to 50 ºC.
  4. Reverse the flow cell inlet and outlet tubes.
  5. Start the flow at a very low rate, ensuring that the back pressure remains below the maximum rating of 1000 psi for the UV flow cell.
  6. Flush the flow cell until the clog is removed.

ADDITIONAL INFORMATION

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