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How to measure sample aspiration with ACQUITY SM-FTN on H-Class, I-Class, and Arc systems - WKB24504

Article number: 24504

OBJECTIVE or GOAL

  • Perform a sample aspiration test on ACQUITY systems with a Sample Manager – Flow Through Needle (SM-FTN).
    • This procedure may be used to troubleshoot the SM-FTN sample withdrawal flow path.
    • This procedure requires a calibrated balance capable of reading to tenth of a milligram. 

ENVIRONMENT

  • ACQUITY APC
  • ACQUITY APC Sample Manager - FTN
  • ACQUITY Arc
  • ACQUITY Arc Premier
  • ACQUITY Arc Sample Manager FTN-R
  • ACQUITY UPLC H-Class
  • ACQUITY UPLC H-Class Bio
  • ACQUITY UPLC I-Class
  • ACQUITY UPLC H-Class PLUS
  • ACQUITY UPLC H-Class PLUS Bio
  • ACQUITY UPLC Sample Manager - Flow Through Needle PLUS (SM-FTN PLUS)
  • ACQUITY Premier System

PROCEDURE 

  1. Add 500 µL to 1000 µL of water into a max recovery vial and add cap with split septa. 
  2. Weigh vial on a balance and record the weight of the vail to 4 decimal places (W1)  
  3. Perform ten injections of 10 µL from the vial (using a short 1 minute method) 
  4. Weigh the vial after completion (W2) 
  5. Determine if the measurement is within an acceptable aspiration of +/- 0.2 µL from 10.0 µL (9.8 µL to 10.2 µL). 
    • [(W1 - W2)] / 10 X 1000 = mg of water withdrawn per injection = µL per injection

ADDITIONAL INFORMATION

id24504, A-30SM, UPAQSM, UPARBQSM, UPARBSMFTN, UPARCH30, UPARHQSM, UPARPFTN, UPARPQSM, UPARPTUV, UPARQSM, UPARSMFTN, UPIBSM, UPIBSM+, UPISMFL, UPISMFL+, UPISMFTIVD, UPISMFTN, UPISMFTN, UPISMFTN+, UPPDAARCB, UPPFTN, UPPSMFL, UPQSM, UPQSM+, UPQSMBIO, UPQSMBIO+, UPSMFTN, UPSMFTN, UPSMFTN+, UPSMFTN+, UPSMFTNB+, UPSMFTNBIO

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