How to measure sample aspiration with ACQUITY SM-FTN on H-Class, I-Class, and Arc systems - WKB24504

OBJECTIVE or GOAL

• Perform a sample aspiration test on ACQUITY systems with a Sample Manager – Flow Through Needle (SM-FTN).
  • This procedure may be used to troubleshoot the SM-FTN sample withdrawal flow path.
  • This procedure requires a calibrated balance capable of reading to tenth of a milligram.　

ENVIRONMENT

• ACQUITY APC
• ACQUITY APC Sample Manager - FTN
• ACQUITY Arc
• ACQUITY Arc Premier
• ACQUITY Arc Sample Manager FTN-R
• ACQUITY UPLC H-Class
• ACQUITY UPLC H-Class Bio
• ACQUITY UPLC I-Class
• ACQUITY UPLC H-Class PLUS
• ACQUITY UPLC H-Class PLUS Bio
• ACQUITY UPLC Sample Manager - Flow Through Needle PLUS (SM-FTN PLUS)
• ACQUITY Premier System

PROCEDURE　

1. Add 500 µL to 1000 µL of water into a max recovery vial and add cap with split septa.　
2. Weigh vial on a balance and record the weight of the vail to 4 decimal places (W1)　　
3. Perform ten injections of 10 µL from the vial (using a short 1 minute method)　
4. Weigh the vial after completion (W2)　
5. Determine if the measurement is within an acceptable aspiration of +/- 0.2 µL from 10.0 µL (9.8 µL to 10.2 µL).　
   • [(W1 - W2)] / 10 X 1000 = mg of water withdrawn per injection = µL per injection

ADDITIONAL INFORMATION