How to measure sample aspiration with ACQUITY SM-FTN on H-Class, I-Class, and Arc systems - WKB24504

OBJECTIVE or GOAL

• Perform a sample aspiration test on ACQUITY systems with a Sample Manager – Flow Through Needle (SM-FTN).
  • This procedure may be used to troubleshoot the SM-FTN sample withdrawal flow path.
  • This procedure requires a calibrated balance capable of reading to tenth of a milligram.　

ENVIRONMENT

• ACQUITY H-Class SM-FTN
• ACQUITY I-Class SM-FTN
• ACQUITY Arc SM-FTN-R

PROCEDURE

1. Select a single vial location, labeled with marker on glass.　
   • Must use Max recovery vials, with caps and spilt septa　
2. Add 800 µL to 1000 µL of water into the vial　
3. Using gloves or a tweezer, pick up the vial by the cap and weigh it on the balance.　
4. Record the weight of the vial out to the fifth decimal. 　
5. Use flow rate of 0.5 to 1.0 mL/min using solvent A isocratically.　
6. Perform ten injections of 10 µL from the vial.　
7. Weigh the vial after completion.　
8. Determine if the measurement is within an acceptable aspiration of +/- 0.2 µL from 10.0 µL (9.8 µL to 10.2 µL).　
   • [(W1 - W2)] / 10 X 1000 = mg of water withdrawn per injection = µL per injection

ADDITIONAL INFORMATION