How to measure sample aspiration with ACQUITY SM-FTN on H-Class, I-Class, and Arc systems - WKB24504
Article number: 24504
OBJECTIVE or GOAL
- Perform a sample aspiration test on ACQUITY systems with a Sample Manager – Flow Through Needle (SM-FTN).
- This procedure may be used to troubleshoot the SM-FTN sample withdrawal flow path.
- This procedure requires a calibrated balance capable of reading to tenth of a milligram.
ENVIRONMENT
- ACQUITY APC
- ACQUITY APC Sample Manager - FTN
- ACQUITY Arc
- ACQUITY Arc Premier
- ACQUITY Arc Sample Manager FTN-R
- ACQUITY UPLC H-Class
- ACQUITY UPLC H-Class Bio
- ACQUITY UPLC I-Class
- ACQUITY UPLC H-Class PLUS
- ACQUITY UPLC H-Class PLUS Bio
- ACQUITY UPLC Sample Manager - Flow Through Needle PLUS (SM-FTN PLUS)
- ACQUITY Premier System
PROCEDURE
- Add 500 µL to 1000 µL of water into a max recovery vial and add cap with split septa.
- Weigh vial on a balance and record the weight of the vail to 4 decimal places (W1)
- Perform ten injections of 10 µL from the vial (using a short 1 minute method)
- Weigh the vial after completion (W2)
- Determine if the measurement is within an acceptable aspiration of +/- 0.2 µL from 10.0 µL (9.8 µL to 10.2 µL).
- [(W1 - W2)] / 10 X 1000 = mg of water withdrawn per injection = µL per injection
ADDITIONAL INFORMATION
id24504, A-30SM, UPAQSM, UPARBQSM, UPARBSMFTN, UPARCH30, UPARHQSM, UPARPFTN, UPARPQSM, UPARPTUV, UPARQSM, UPARSMFTN, UPIBSM, UPIBSM+, UPISMFL, UPISMFL+, UPISMFTIVD, UPISMFTN, UPISMFTN, UPISMFTN+, UPPDAARCB, UPPFTN, UPPSMFL, UPQSM, UPQSM+, UPQSMBIO, UPQSMBIO+, UPSMFTN, UPSMFTN, UPSMFTN+, UPSMFTN+, UPSMFTNB+, UPSMFTNBIO