What is Waters Magic Mix/AAA Wash? - WKB238676

ENVIRONMENT

• Alliance HPLC Systems
• ACQUITY Arc Systems
• ACQUITY UPLC Systems
• ACQUITY UPLC H-Class
• ACQUITY UPLC I-Class
• ACQUITY UPLC M-Class
• nanoACQUITY UPLC

ANSWER

Waters Magic Mix/AAA Wash is a general cleaning solution used to remove contaminants from internal HPLC and UPLC system components. The composition of the Magic Mix/AAA Wash is equal parts 25:25:25:25 MS/HPLC-grade H2O/ACN/MeOH/IPA plus 0.1 to 2% formic acid (typically).

ADDITIONAL INFORMATION

General Cleaning Procedure:  

1. Remove the column and replace it with a union.
2. Put all lines from the ACQUITY/Alliance into the alcohol or Magic Mix, which is 25:25:25:25 water/isopropyl alcohol/methanol/acetonitrile and 0.2% formic acid (can go up to 2% formic acid depending on how dirty/contaminated the system is).
3. Flush the system by priming for five minutes, and then pump for at least two hours (preferably overnight). Use restrictor to give the system some back pressure.
4. Prime and flush with fresh LC/MS-grade or Milli-Q water. 
5. Put on fresh LCMS-grade solvents that are used for the LC-MS analysis. If the solvent bottles have not been cleaned recently, use the alcohol mix to clean out the bottles, and then rinse them well with fresh solvent.