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Waters

What is Waters Magic Mix/AAA Wash? - WKB238676

Article number: 238676

ENVIRONMENT

  • Alliance HPLC Systems
  • ACQUITY Arc Systems
  • ACQUITY UPLC Systems
  • ACQUITY UPLC H-Class
  • ACQUITY UPLC I-Class
  • ACQUITY UPLC M-Class
  • nanoACQUITY UPLC

ANSWER

Waters Magic Mix/AAA Wash is a general cleaning solution used to remove contaminants from internal HPLC and UPLC system components. The composition of the Magic Mix/AAA Wash is equal parts 25:25:25:25 MS/HPLC-grade H2O/ACN/MeOH/IPA plus 0.1 to 2% formic acid (typically).

ADDITIONAL INFORMATION

General Cleaning Procedure:  

  1. Remove the column and replace it with a union.
  2. Put all lines from the ACQUITY/Alliance into the alcohol or Magic Mix, which is 25:25:25:25 water/isopropyl alcohol/methanol/acetonitrile and 0.2% formic acid (can go up to 2% formic acid depending on how dirty/contaminated the system is).
  3. Flush the system by priming for five minutes, and then pump for at least two hours (preferably overnight or extend the cleaning for a few days on systems with bad contamination). Use restrictor to give the system some back pressure.
  4. Prime and flush with fresh LC/MS-grade or Milli-Q water. 
  5. Put on fresh LCMS-grade solvents that are used for the LC-MS analysis. If the solvent bottles have not been cleaned recently, use the alcohol mix to clean out the bottles, and then rinse them well with fresh solvent. 

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