Alliance 2695 shows many chromatogram spikes - WKB228408

SYMPTOMS

• Consecutive spikes in chromatogram baseline

ENVIRONMENT

• Alliance HPLC System
• 2695 Separations Module
• ARC HPLC
• ACQUITY ARC

CAUSE

Flow path/solvent lines dirty from from usage (different solvent and/or samples)

FIX or WORKAROUND

1. Prepare Magic Mix (25% each of H₂O; MeOH, ACN, IPA) and add up to 10% formic acid.
2. Remove the column from the system.
3. Prime the solvent manager and the needle wash with Magic Mix.
4. Apply a flow of 0.5 mL/min and leave Magic Mix on the system overnight.
5. Prime all lines to 100% H₂O.
6. Apply a flow of 0.5 mL/min for 30 minutes.
7. Prepare new solvents and set up your system as needed.
8. Check the baseline.

ADDITIONAL INFORMATION