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Alliance 2695 shows many chromatogram spikes - WKB228408

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Article number: 228408

SYMPTOMS

  • Consecutive spikes in chromatogram baseline

clipboard_e3de4d848c49b4feba0ecd6c19576b937.png

ENVIRONMENT

  • Alliance HPLC System
  • 2695 Separations Module
  • ARC HPLC
  • ACQUITY ARC

CAUSE

Flow path/solvent lines dirty from from usage (different solvent and/or samples)

FIX or WORKAROUND

  1. Prepare Magic Mix (25% each of H2O; MeOH, ACN, IPA) and add up to 10% formic acid.
  2. Remove the column from the system.
  3. Prime the solvent manager and the needle wash with Magic Mix.
  4. Apply a flow of 0.5 mL/min and leave Magic Mix on the system overnight.
  5. Prime all lines to 100% H2O.
  6. Apply a flow of 0.5 mL/min for 30 minutes.
  7. Prepare new solvents and set up your system as needed.
  8. Check the baseline.

ADDITIONAL INFORMATION

 

id228408, 2690, 2690D, 2690DUPGD, 2690UP, 2695, 2695D, 2695DE, 2695DEB, 2695DUP, 2695E, 2695EB, 2695INERT, 2695UP, A-10SSM, ALLCOLCLR, ALLCOLHTR, ALLCOLHTRB, isopropanol, methanol, UPAQSM, UPARBQSM, UPARBSMFTN, UPARCH30, UPARHQSM, UPARQSM, UPARSMFTN, UPPDAARCB

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