What are the guidelines for RI detector purge time when using a new column and mobile phase? - WKB224716
Article number: 224716
ENVIRONMENT
- Alliance HPLC System
- 2695 Separations Module
- Arc HPLC System
- ACQUITY Arc
- ACQUITY UPLC
- ACQUITY UPLC H-Class
- 2414 Refractive Index Detector
- 2998 Photodiode Array Detector
- ACQUITY UPLC RI Detector
ANSWER
- Perform a RI detector purge after passing at least 20 times the column capacity through the column.
- Incomplete column equilibration can affect the chromatogram, even with purging.
- When using a new column or changing the composition of the mobile phase, be sure to fully equilibrate the column.
- Depending on the type of column and mobile phase, it may be necessary to pass 20 times or more of the column capacity.
- Purge the RI detector for at least 30 minutes.
ADDITIONAL INFORMATION
id224716, 2414, 2414B, 2690, 2690D, 2690DUPGD, 2690UP, 2695, 2695D, 2695DE, 2695DEB, 2695DUP, 2695E, 2695EB, 2695INERT, 2695UP, 2998, 2998B, 2998SFC, A-10PDA, A-10RI, A-10SSM, A-30RI, eluent, EMP2LIC, EMP2OPT, EMP2SW, EMP3GC, EMP3LIC, EMP3OPT, EMP3SW, EMPGC, EMPGPC, EMPLIC, EMPOWER2, EMPOWER3, EMPSW, SUP, UPAQSM, UPARBQSM, UPARBSMFTN, UPARCH30, UPARHQSM, UPARQSM, UPARSMFTN, UPASMFTN, UPBSMIVD, UPIBSMIVD, UPLCCOLHTR, UPNBINARY, UPPDAARCB, UPQSM, UPQSM+, UPQSMBIO, UPQSMBIO+, UPRI, UPRIARC, UPSMFTN, UPSMFTN+, UPSMFTNB+, UPSMFTNBIO, UPSMIVD, UPSMPMGR