What are the guidelines for RI detector purge time when using a new column and mobile phase? - WKB224716
Article number: 224716
ENVIRONMENT
- Alliance HPLC System
- 2695 Separations Module
- Arc HPLC System
- ACQUITY Arc
- ACQUITY UPLC
- ACQUITY UPLC H-Class
- 2414 Refractive Index Detector
- 2998 Photodiode Array Detector
- ACQUITY UPLC RI Detector
ANSWER
- Perform a RI detector purge after passing at least 20 times the column capacity through the column.
- Incomplete column equilibration can affect the chromatogram, even with purging.
- When using a new column or changing the composition of the mobile phase, be sure to fully equilibrate the column.
- Depending on the type of column and mobile phase, it may be necessary to pass 20 times or more of the column capacity.
- Purge the RI detector for at least 30 minutes.
ADDITIONAL INFORMATION