How is needle cleaned on ACQUITY UPLC Sample Manager using a strong wash solvent and a weak wash solvent? - WKB205077

ENVIRONMENT

• ACQUITY UPLC Sample Manager

ANSWER

Weak wash solvent: Mobile phase composition, initial composition recommended for gradient (salt not included)
Strong wash solvent: Solvent that is strong enough to dissolve the sample (salt not included). Example: 100% organic solvent

- Wash port
Left: Wash the inside of the needle with a weak solvent.
Right: Wash the inside/outside of the needle with a strong/weak solvent.

■ Left port
① Wash the inside of the needle with a weak solvent.
■ Right port
② Wash the inside of the needle with a strong solvent from top, while pushing up with a weak solvent from bottom at the same time.
* The outside of the needle (the gap between the drilling needle) is cleaned while the two solvents are mixed, and the liquids are drained to waste from top.
③ Wash the inside of the needle with a weak solvent.
④ Drain the liquid remaining in the wash port to waste.
* ① occurs before sample aspiration and ①-④ occur after injection.

ADDITIONAL INFORMATION

You can obtain a higher cleaning effect by increasing the volume of the strong/weak solvent.
The setting is made in "Instrument Method (Empower)". The ratio of weak wash solvent to strong wash solvent should be approximately 3:1.