What is an injection peak? - WKB200815
ENVIRONMENT
- Alliance HPLC System
- 2695 Separations Module
- ACQUITY UPLC
- ACQUITY UPLC H-Class
- ACQUITY Arc
- ACQUITY UPLC PDA Detector
- ACQUITY UPLC PDA eλ Detector
- ACQUITY UPLC Tunable UV Detector
- ACQUITY UPLC FLR Detector
- 2998 Photodiode Array Detector
- 2489 UV/Vis Detector
- 2996 PDA Detector
- 2475 Multi-Wavelength Fluorescence Detector
ANSWER
"Injection peak" refers to the peak (signal) that appears near t0, which comes from the sample and its injection operation.
(t0: the retention time of solute that is not retained and passes through the column at the same speed as the mobile phase)
The injection peak intensity is mainly determined by the following factors.
- Dissolving solvent for sample
- Contamination from flow paths where mobile phases come into contact with valves, needles, columns, tubes, and so on
- Impurities in the sample
- Acids, bases, salts in the sample
The injection peak does not always appear like a Gaussian peak, and the peak may swing positively or negatively in a short time.
Multiple peaks may be detected due to the above factors.
The injection peaks observed in UV and FL chromatograms may depend on wavelength.
When caused by contamination in the flow path, the shape and intensity of the injection peak may not be reproducible.
The injection peak may be considered as t0, but it is generally recommended to use the optimal t0 marker for each separation mode you use.
The reproducibility of the injection peak and the peak of the target component are not always related.
ADDITIONAL INFORMATION
id200815, A-30FL, A-30PDA, A-30UV, ALLCOLCLR, ALLCOLHTR, ALLCOLHTRB, eluent, UPAPDA, UPAQSM, UPARBQSM, UPARBSMFTN, UPARCH30, UPARHQSM, UPARPTUV, UPARQSM, UPARSMFTN, UPBINARY, UPC2PDA, UPFLRARC, UPFLRDET, UPPDA, UPPDAARC, UPPDAARCB, UPPDAARCB, UPPDA-E, UPPDA-L, UPPDALTC, UPPDATC, UPPFLR, UPPPDA, UPPTUV, UPQSM, UPQSM+, UPQSMBIO, UPQSMBIO+, UPSMFTN, UPSMFTN+, UPSMFTNB+, UPSMFTNBIO, UPTUV, UPTUVARC, UPTUVARCB, UPTUV-E, UPTUVTC