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How to flush blocked UPLC flow cell? - WKB19946

Article number: 19946

OBJECTIVE or GOAL

Flush a blocked UPLC flow cell, which is characterized by high pressure in the flow cell.

ENVIRONMENT

  • ACQUITY UPLC TUV
  • ACQUITY UPLC PDA
  • 2996 PDA Detector

PROCEDURE

  1. Unscrew the outlet and inlet lines of the flow cell
  2. Connect the mobile phase tubing to the outlet of the flow cell
  3. Use suitable solvent (methanol, water, acetonitrile, tetrahydrofuran, DMSO, DMF) to backflush the flow rate with a low flow rate. Note that the pressure of the flow cell shall not exceed 1000 psi.
    Backflush_Flowcell.jpg
  4. If backflushing doesn’t work, try unscrewing the metal fitting at the inlet at the rear of the flow cell.
    unscrewinletfitting.jpg
  5. A sudden drop of pressure indicates that the flow cell has been flushed successfully.

ADDITIONAL INFORMATION

 

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