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How to flush blocked UPLC flow cell? - WKB19946

Article number: 19946

OBJECTIVE or GOAL

How to back flush a flow cell, which is characterized by high pressure in the flow cell.

ENVIRONMENT

  • 2475 Multi-Wavelength Fluorescence Detector
  • 2487 Dual-Wavelength Absorbance Detector
  • 2488 Multichannel Absorbance Detector
  • 2489 UV/Vis Detector
  • 2996 PDA Detector
  • 2998 Photodiode Array Detector
  • ACQUITY UPLC Tunable UV Detector
  • ACQUITY UPLC PDA Detector
  • ACQUITY UPLC PDA eλ Detector
  • ACQUITY UPLC FLR Detector

PROCEDURE

  1. Unscrew the outlet and inlet lines of the flow cell
  2. Connect the mobile phase tubing to the outlet of the flow cell
  3. Use suitable solvent (methanol, water, acetonitrile, tetrahydrofuran, DMSO, DMF) to backflush the flow rate with a low flow rate. Note that the pressure of the flow cell shall not exceed 1000 psi.
    Backflush_Flowcell.jpg

ADDITIONAL INFORMATION

For UPLC flow cells, the following procedure can be used:

 

  1. If backflushing doesn’t work, try unscrewing the metal fitting at the inlet at the rear of the flow cell.
    unscrewinletfitting.jpg
  2. A sudden drop of pressure indicates that the flow cell has been flushed successfully.

eluent

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