How to flush blocked UPLC flow cell? - WKB19946
Article number: 19946
OBJECTIVE or GOAL
How to back flush a flow cell, which is characterized by high pressure in the flow cell.
ENVIRONMENT
- 2475 Multi-Wavelength Fluorescence Detector
- 2487 Dual-Wavelength Absorbance Detector
- 2488 Multichannel Absorbance Detector
- 2489 UV/Vis Detector
- 2996 PDA Detector
- 2998 Photodiode Array Detector
- ACQUITY UPLC Tunable UV Detector
- ACQUITY UPLC PDA Detector
- ACQUITY UPLC PDA eλ Detector
- ACQUITY UPLC FLR Detector
PROCEDURE
- Unscrew the outlet and inlet lines of the flow cell
- Connect the mobile phase tubing to the outlet of the flow cell
- Use suitable solvent (methanol, water, acetonitrile, tetrahydrofuran, DMSO, DMF) to backflush the flow rate with a low flow rate. Note that the pressure of the flow cell shall not exceed 1000 psi.
ADDITIONAL INFORMATION
For UPLC flow cells, the following procedure can be used:
- If backflushing doesn’t work, try unscrewing the metal fitting at the inlet at the rear of the flow cell.
- A sudden drop of pressure indicates that the flow cell has been flushed successfully.
id19946, 2475, 2475B, 2487, 2489, 2489B, 2489SFC, A-10FL, A-10UV, A-30FL, A-30PDA, A-30UV, Dimethyl sulfoxide, eluent, UPAPDA, UPARPPDA, UPARPTUV, UPC2PDA, UPFLRARC, UPFLRDET, UPIBSM, UPIBSM+, UPISMFL, UPISMFL+, UPISMFTIVD, UPISMFTN, UPISMFTN+, UPPDA, UPPDAARC, UPPDAARCB, UPPDA-E, UPPDA-L, UPPDALTC, UPPDATC, UPPFLR, UPPPDA, UPPTUV, UPQSM, UPQSM+, UPQSMBIO, UPQSMBIO+, UPSMFTN, UPSMFTN+, UPSMFTNB+, UPSMFTNBIO, UPTUV, UPTUVARC, UPTUVARCB, UPTUV-E, UPTUVTC