"Failed to Calibrate system volume" error message on ACQUITY UPLC Sample Manager - WKB195843

SYMPTOMS

• Error message "Failed to Calibrate system volume" when characterizing the needle and loop
• 10-µL loop, 30-µL needle, 50-µL sample syringe 
• VDD calibration and all other leak tests (sample syringe, wash syringe, needle seal) pass

ENVIRONMENT

• ACQUITY UPLC Sample Manager 

CAUSE

Incorrect sample loop and/or sample syringe installed. The 50-µL sample syringe volume is to low for characterizing needle and loop.

FIX or WORKAROUND

1. Install the 100-µL sample syringe (700002570 - Syringe, 100 µL, HP).
2. Or, install smaller sample loop (430001516 - Sample Loop, 1 µL; 430001264 - Sample Loop, 2 µL; 430001311 - Sample Loop, 5 µL).

ADDITIONAL INFORMATION