Skip to main content
Waters

No chromatographic peaks with ACQUITY I-Class - WKB19542

Article number: 19542

SYMPTOMS

  • No chromatographic peaks
  • No visible leaks
  • No errors
  • System pressure is normal
  • Z axis was calibrated and it passes the needle seal characterization
  • Fails the sample syringe leak test

ENVIRONMENT

  • ACQUITY I-Class
  • ACQUITY H-Class

CAUSE

Air bubbles in the purge line and in the sample syringe

FIX or WORKAROUND

  1. In the console, go to the FTN.
  2. From the Control drop-down menu, select Prime.
  3. Set Wash Solvent to 60 sec.
  4. Set Purge Solvent to 50 cycles.
  5. When priming completes (approximately 25 minutes), run a leak test on the sample syringe (Maintain drop-down menu > Leak test > Start) to confirm that it passes.

ADDITIONAL INFORMATION

Not able to find a solution? Click here to request help.