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How to program ACQUITY SM-FTN Auto-Additions in Empower - WKB1416

Article number: 1416

OBJECTIVE or GOAL

  • Program Auto Additions in Empower Run Samples and program "delay" to function in the SM-FTN instrument editor.

ENVIRONMENT

  • Empower 3
  • ACQUITY SM-FTN instrument editor "Advance" option

PROCEDURE

  1. In the SM-FTN instrument editor, from the General tab, click "Advanced".
  2. Select both "Mix Cycles" and "Mix Stroke Volume", and input a value (0-99) in the fields. "Automatic = 0"
  3. Save the Instrument Method and the Method Set to be used during acquisition.
  4. Open Empower Run Samples.
  5. Right-click in Auto Additions and select the Auto-Additions Editor.
  6. Use the Auto-Additions Editor to program the Plate/Well and Inj Vol (uL) with Delay in minutes.
  7. Complete the remaining Sample Set information.

ADDITIONAL INFORMATION

  • You may observe the known issue of the Auto-Additions delay hold time not being accepted or carried out. 
  •  If no mix cycles are programmed, the auto dilution delay time will be ignored. Workaround: Program a brief mixing cycle by volume; this should trigger the delay time. 

Example:

Run Samples Auto-Additions Editor: Pickup from three vials with respective volumes and delay by 5 mins after mixing.
Example1.png

SM-FTN instrument editor Advance selection showing two cycles to mix the samples by displacing 10 uL inside the needle
Example2.png

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