No peaks found in chromatographic runs - WKB12976

SYMPTOMS

• No peaks in the report after injecting samples
• Baseline is erratic and drops to zero
• No leaks found on the system
• With column disconnected and flow running, see no eluant come out
• Solvent bottle's intake line is not at the bottom of the bottle

ENVIRONMENT

• ACQUITY UPLC H-Class
• MassLynx 4.1

CAUSE

Air bubble in the solvent line

FIX or WORKAROUND

1. Prime solvent lines.

ADDITIONAL INFORMATION