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Waters

No peaks observed on ACQUITY UPLC H-Class - WKB12552

Article number: 12552

SYMPTOMS

  • No peak observed when making an injection using Empower 3
  • Peak observed when sample is manually pushed through the flow cell

ENVIRONMENT

  • ACQUITY UPLC H-Class
  • Empower 3

CAUSE

The mobile phase solvents are 100% water, more than one week old, and/or of unknown composition.

FIX or WORKAROUND

  1. Prepare fresh solvents.
  2. Prime each line for five minutes.
  3. Prime, purge, and wash solvents; 30-50 cycles for purge solvent and 60 seconds for wash solvent.
  4. Characterize the needle seal.
  5. Make a test injection.

ADDITIONAL INFORMATION

 

 

id12552, eluent, EMP2LIC, EMP2OPT, EMP2SW, EMP3GC, EMP3LIC, EMP3OPT, EMP3SW, EMPGC, EMPGPC, EMPLIC, EMPOWER2, EMPOWER3, EMPSW, SUP, UPBINARY, UPQSM, UPQSM+, UPQSMBIO, UPQSMBIO+, UPSMFTN, UPSMFTN+, UPSMFTNB+, UPSMFTNBIO

 

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