No peaks observed on ACQUITY UPLC H-Class - WKB12552
Article number: 12552
SYMPTOMS
- No peak observed when making an injection using Empower 3
- Peak observed when sample is manually pushed through the flow cell
ENVIRONMENT
- ACQUITY UPLC H-Class
- Empower 3
CAUSE
The mobile phase solvents are 100% water, more than one week old, and/or of unknown composition.
FIX or WORKAROUND
- Prepare fresh solvents.
- Prime each line for five minutes.
- Prime, purge, and wash solvents; 30-50 cycles for purge solvent and 60 seconds for wash solvent.
- Characterize the needle seal.
- Make a test injection.
ADDITIONAL INFORMATION
id12552, eluent, EMP2LIC, EMP2OPT, EMP2SW, EMP3GC, EMP3LIC, EMP3OPT, EMP3SW, EMPGC, EMPGPC, EMPLIC, EMPOWER2, EMPOWER3, EMPSW, SUP, UPBINARY, UPQSM, UPQSM+, UPQSMBIO, UPQSMBIO+, UPSMFTN, UPSMFTN+, UPSMFTNB+, UPSMFTNBIO