Negative peaks on 2475 or 2489 detector - WKB115919
Article number: 115919
SYMPTOMS
- Negative baseline/peaks throughout the chromatogram
- Compression Check successful
- New flow cell
ENVIRONMENT
- Alliance HPLC system
- 2695 Separations Module
- 2475 Multi-Wavelength Fluorescence Detector
- 2489 UV/VIS Detector
CAUSE
Bad mobile phase
FIX or WORKAROUND
Replace old solvents with fresh mobile phases.
ADDITIONAL INFORMATION
id115919, 2475, 2475B, 2690, 2690D, 2690DUPGD, 2690UP, 2695, 2695D, 2695DE, 2695DEB, 2695DUP, 2695E, 2695EB, 2695INERT, 2695UP, A-10FL, A-10SSM, ALLCOLCLR, ALLCOLHTR, ALLCOLHTRB, eluent