ACQUITY UPLC system over pressure - WKB113364

SYMPTOMS

• The system pressure is over the MAX limit with flow rate established in analytical method

ENVIRONMENT

• ACQUITY UPLC I-Class
• ACQUITY UPLC H-Class
• ACQUITY UPLC 

CAUSE

Clog in system flow path 

FIX or WORKAROUND

1. Find the clogged tubings and replace them

   1. Remove the probe tubing from the MS divert valve.

   2. Remove the LC tubing from the MSdivert valve.

   3. Remove the other end of the LC tubing from the UV detector output.

   4. Remove the tubing from the UV detector input.

   5. Remove the other end of the UV detector input tubing from the column or column manager.

   6. If a column manager is present, switch it to bypass. 

   7. If the issue is present only when not in bypass, or if not column manager prsent, remove the column.

   8. If a column manager is present, remove the active preheater from the inlet valve of the manager.

   9. Remove the active preheater or column manager input tubing from the injection valve.

   10. Switch the injection valve to both the load and inject positions.

   11. Remove the pump outlet tubing from the inject valve.

   12. Remove the pump outlet tubing from the mixer.

   13. Remove the mixer.

   14. Remove the mixer inlet tubing from the vent valve output.

   15. Remove the pump head outlet tubing from the vent valve inlet.

   16. Remove the pump head outlet tubing from the accumlator check valve.

       By this point, you will either have found the blockage or isolated it to the pump head

2. Flush the system with Magic Mix (25% water, 25% methanol, 25% acetonitrile, 25% isopropanol).
3. Replace the column.

ADDITIONAL INFORMATION

Bacteria will grow in pure aqueous mobile phase if the system is shut down for more than a week.