Wavy baseline at low UV wavelengths - WKB1035
Article number: 1035
SYMPTOMS
- Wavy/noisy baseline on ACQUITY instruments at low UV wavelengths
ENVIRONMENT
- ACQUITY UPLC H-Class
- ACQUITY UPLC Tunable UV Detector (TUV)
- ACQUITY UPLC PDA Detector
CAUSE
Baseline anomalies caused by refractive index changes are enhanced at low UV.
FIX or WORKAROUND
- If using a 30cm Column Heater, insulate lines in and out of the column.
ADDITIONAL INFORMATION
Factors that affect RI:
- Temperature
- Dissolved gases
- Electronic pulses
- Environmental interference