Skip to main content
Waters

Not all of the doubly charged isotopes show up in the processed spectrum in UNIFI - WKB27022

Article number: 27022

SYMPTOMS

  • Not all of the doubly charged isotopes show up in the processed spectrum
  • The singly charged isotopes are fine
  • The doubly charged isotope peaks are there in Investigate

ENVIRONMENT

  • UNIFI
  • Peptide data
  • Small-molecule data

CAUSE

The analysis method was not set to use doubly charged data.

FIX or WORKAROUND

  1. In the analysis method, go to Analysis Specific Settings and select Adducts.

UNI adducts analysis method.PNG

  1. Increase the maximum allowed absolute charge for adduct combinations to two if there are doubly charged ions (three if there are triply charged ions in the sample).
  2. Select "Allow cross adduct combinations".

UNI adducts 2.PNG

 

4. Alternatively, create a [2H]2+ adduct in the Scientific Library (Modifications > Adducts).This will avoid combinations of Na+ and H+ being generated.

ADDITIONAL INFORMATION

 

SUPUNIFI, UNIFISVR, UNIFISW18, UNIFOPT, UNIFQLIC, UNIFSW17, UNIFSW18, UNIFSW19, UNIFWGLIC, UNIFWKLIC

Not able to find a solution? Click here to request help.