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Processing Peptide Mapping Data uses all memory - WKB108869

Article number: 108869

SYMPTOMS

  • Peptide mapping containing numerous disulfide bonds and missed cleavages can generate many
    fragment ions, which results in a memory error.

ENVIRONMENT

  • UNIFI 1.9.4
  • UNIFI 1.9.9

CAUSE

  • CRI-138 (INFULA-1201)

FIX or WORKAROUND

Create a map of potential disulfide bonded regions of a protein by performing a mass
spectrometry search with and without disulfide bonds. Then, add one or two disulfides to the
protein and gradually add the information to the peptide map.

For complex disulfide patterns with more than 2 disulfides per peptide, consider removing peptide chains from the sequence, and then create a custom Cys modification comprised of that chain, using a look ahead and/or look behind sequence in the modifier to specifically direct that chain to a specific Cys residue

ADDITIONAL INFORMATION

 

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