ProteinLynx Global Server (PLGS) search engine log or Progenesis QI for Proteomics iadbs log reports "insufficient Pass 0 matches for calibration" - WKB31556

SYMPTOMS

• ProteinLynx Global Server (PLGS) search engine log reports "insufficient Pass 0 matches for calibration"
• Reviewing the experimental record in the .raw data folder reveals that the calibration was not applied (i.e., no polynomial curve was recorded on the Function 1/2/3 lines)

Example from a header.txt file that produces the "insufficient Pass 0 matches for calibration" report in the PLGS search engine log:

$$ Calibration File:
$$ Cal Function 1: 0.000000000000000e0,1.000000000000000e0,T1
$$ Cal Function 2: 0.000000000000000e0,1.000000000000000e0,T1
$$ Cal Function 3: 0.000000000000000e0,1.000000000000000e0,T1

Example from a header.txt file that does not produce the "insufficient Pass 0 matches for calibration" report in the search engine log:

$$ Calibration File:
$$ Cal Function 1: -3.640508329687166e-3,1.000011633228105e0,1.476993595932374e-5,-7.510650812437795e-7,1.798164604968282e-8,-1.622170641677714e-10,T1
$$ Cal Function 2: -3.640508329687166e-3,1.000011633228105e0,1.476993595932374e-5,-7.510650812437795e-7,1.798164604968282e-8,-1.622170641677714e-10,T1
$$ Cal Function 3: -3.640508329687166e-3,1.000011633228105e0,1.476993595932374e-5,-7.510650812437795e-7,1.798164604968282e-8,-1.622170641677714e-10,T1

ENVIRONMENT

• ProteinLynx Global Server
• MassLynx SCN 983

CAUSE

The use of files from a previous SCN

FIX or WORKAROUND

1. Create a new project from default.
2. Create new Tune Page, MS Method, Inlet Method, and Sample List files.
3. Reacquire the data.

ADDITIONAL INFORMATION