No concentrations of analytes or internal standards being calculated in TargetLynx - WKB85611

SYMPTOMS

• not able to get any concentrations to be calculated in the TargetLynx results file for "Analytes" or "Internal Standards" samples
• "standards" are able to be found and have concentrations calculated
• calibration curve is created
• peaks for the compounds are present in the MassLynx chromatogram traces
• peaks for the internal standard compounds are not present in the chromatogram traces

ENVIRONMENT

• MassLynx 4.2 SCN 966
• TargetLynx

CAUSE

Compounds are set to use Internal (relative - use internal standards) and there are no corresponding internal standard peak found in the data traces.

Internal Standards compounds are set to External (absolute - no Internal Standard)

FIX or WORKAROUND

1. Check sample preparation or determine why no internal standard should be present in those samples or
2. Edit the processing method to use External (absolute - no internal standards) instead of Internal (relative - use internal standards).
3. For missing Internal Standard, Analysis Response Type in Compound Properties needs to be set to Internal rather than External.

ADDITIONAL INFORMATION