How to determine peak asymmetry and efficiency in TargetLynx - WKB71002

OBJECTIVE or GOAL

Determine peak asymmetry and efficiency in TargetLynx. 

ENVIRONMENT

• TargetLynx
• MassLynx

PROCEDURE

1. Open a TargetLynx file, right-click on the table, and select "change column order". This gives an overview of how the table is constructed.
   
2. From the left, select "Peak Asymmetry". You can move this to the right by clicking "add". The peak asymmetry is defined as the tail width (b) / front width (a).
   This can alternatively be calculated as the Tailing Factor using the formula T=(a+b)/2a. Move Formula1 to the right hand side, double-click and click on "formula". The formula should be set to (Tail Width+Front Width)/(2*Front Width).
   Note: The US Pharmocopea states that you must measure peak width at 10% height, so in the TargetLynx method, under Peak asymmetry, set the Measure At Height (%) value to 10. The values of a and b will then be calculated at 10 % height. 
3. 
   
4. Do the same for efficiency. A second formula can be added for this: 5.54*(Found Peak RT*60/Pk Width)*(Found Peak RT*60/Pk Width).
   These formula columns can be renamed, to "plate-count" for example.
   
   MassLynx does not have the option to have chromatographic peak resolution calculated.

ADDITIONAL INFORMATION