Missing vial or incorrect plate location error message in MassLynx when running samples - WKB280039

SYMPTOMS

• Missing vial or incorrect plate location error message in MassLynx
• MassLynx sample list goes to Pause mode
• Incorrect vial location is specified for one of the samples
• No vial present in the incorrect location

ENVIRONMENT

• MassLynx 4.2

CAUSE

Incorrect vial location specified; no vial present in the incorrect location

FIX or WORKAROUND

1. At the top of the MassLynx page, select Queue.
2. Click on the batch lozenge in the Queue, select Delete Process, and confirm.
3. Unpause the Sample List by clicking on the Pause button on the tool bar at top of the MassLynx page.
4. From the Inlet Method, go to LC > Reset Injector and LC > Reset Communications.
5. From the MS Console, highlight the sample manager in the list on the left-hand side and go to Control > Reset SM. 
6. In the Sample List, specify the correct vial location and save the Sample List.
7. Highlight the lines of the Sample List starting with the line where the correction was made to the end of the list.
8. Click the Run button at the top of the Sample List to restart the batch at the point where it left off or failed. 
9. Click YES to overwrite the files names. (Note: the file was created when the sample was first queued, but there are no data in the file because it did not run.)

ADDITIONAL INFORMATION

If the vial location set in MassLynx is determined to be correct, verify that there is a vial in the specified position and that the sample tray is in the correct location in the sample manager bed. 

When an error such as an incorrect vial location is made on a Sample List, you cannot simply make the change to the Sample List and save it while the batch is still in the Queue. MassLynx will save the changes to the file, but the original version of the Sample List with the incorrect information is still in the Queue and is not updated simply by saving the Sample List. This is why you need to clear the batch from the Queue, correct and save the Sample List, and resubmit it to the Queue.