Using no calibration curve, how do you calculate the concentration in TargetLynx from the concentration of the internal standards? - WKB234165

OBJECTIVE or GOAL

Calculate the concentration of the analyte from its internal standard using no calibration curve.

ENVIRONMENT

• MassLynx
• QuanLynx
• TargetLynx

PROCEDURE

1. Create the component for the Internal Standard in the TargetLynx method.
2. Specify the concentration of the Internal Standard on the Calibration Properties page in the TargetLynx Method, in either of two ways:
   • Set a Concentration of Standard: Level as Fixed and specify Concentration of Standard to that of the Internal Standard.
   • Set a Concentration of Standard: Level to the Conc X column in the sample list. Specify the Concentration of the Internal Standards in this column.
3. Create the component for the Analyte in the TargetLynx method.
4. On the Compound Properties page, set the Response Type to Internal (relative - use internal standard).
5. Set Internal Standard 1 to the appropriate Internal Standard from the Component List.
6. On the Calibration Properties page, set the Polynomial Type to Manual RF.
7. On the Calibration Properties page, set the User RF Value to 1.0.
8. Save the method.

ADDITIONAL INFORMATION

The calculation for Response when using Response Type = Internal (relative - use internal standard) is:

Response = (Area Analyte / Area IS) x Concentration IS

Using a Response Factor (RF) of 1.0 converts this Response value directly to a Concentration value.