QDa MS Data in Empower - Extracting Chromatograms and Spectra - Tip361

OBJECTIVE or GOAL

How to Extract Chromatograms and Spectra While in the Mass Analysis Window

ENVIRONMENT

• Empower
• Empower Tip of the Week #361

PROCEDURE

If you are already familiar with working in the main window of Review, then you know how to extract chromatograms and spectra from your 3D data. Once you are in the Mass Analysis window you can do the same without having to go back to the main window in Review.

STEP 1
If you right-click on a spectrum, you can extract a chromatogram at a particular mass, in this case, 342.05 (figure 1).

STEP 2
The extracted chromatogram appears in the bottom plot as Manual XIC (extracted ion chromatogram) at 342.05 (figure 2). 

STEP 3
If you right-click on a chromatogram, you can extract a spectrum at that particular point in time, in this case, 1.210 minutes (figure 3). 

STEP 4
The extracted spectrum appears in the spectral view as Manual – 1.210 (figure 4). 

STEP 5
There are drop-down lists at the bottom of the window that allow you to extract channels from 3D PDA or QDa data much like you would in the main Review window. We have both PDA and QDa data in this data, therefore, the choices reflect the data collected. If you select one of the PDA choices, the source field will auto-populate. If you select one of the MS choices and you have multiple MS scans, you will select which scan from which the chromatogram will be extracted (figure 5). 

STEP 6
For example, we can extract a single wavelength chromatogram from our 3D PDA data, in this case at 310nm. The chromatogram appears in the bottom plot as Manual – XIC – PDA Spectrum: 310.0 nm (figure 6).

ADDITIONAL INFORMATION