Processing Methods - Process Data with a Processing Method instead of a Method Set - wkb262820

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Article number: 262820

OBJECTIVE or GOAL

Determine when to process data with a Processing Method instead of a Method Set.

ENVIRONMENT

Empower
Empower Tip of the Week #330

PROCEDURE

The type of data you are working with, the number of channels collected, and what needs to be processed will determine whether you can process the data with a Processing Method instead of a Method Set.

The first scenario is single-channel 2D data processed with one Processing Method.

STEP 1
A Sample Set was collected with single-channel UV data, and we want all injections to be processed in the same way (Figure 1).

STEP 2
The Method Set displays the Instrument Method and the Processing Method in the Default Processing Method field (Figure 2).

STEP 3
The Instrument Method shows that the single channel TUV@ 280nm was collected and nothing else (Figure 3).

STEP 4
We can process the Sample Set in one of three ways, which all produce the same set of Results (Figure 4).

With the Acquisition Method Set
With a Specified Method Set where we select the Method Set that was used to collect the data
With the Processing Method specified in the Method Set

The next scenario is multi-channel 2D data.

STEP 5
In addition to the TUV channel, we have enabled System Pressure for the Binary Solvent Manager (BSM) to be collected with every injection (Figure 5).

STEP 6
If we process the Sample Set with the Method Set as shown in Figure 2, both the TUV channel and the System Pressure channel will be processed. Because the System Pressure channel is typically used as a diagnostic, we may want to process only the TUV channel. Therefore, we can select the TUV channel and the appropriate Processing Method in the channel table of the Method Set (Figure 6).

STEP 7
We have now enabled a second TUV channel to be collected with every injection. If we need to process each channel with its own specified Processing Method, this can be done by selecting each channel and its associated Processing Method into the channel table of the Method Set (Figure 7).

STEP 8
The same would hold true if we were collecting multi-channel data from different detectors, such as UV and RI (Figure 8).

These scenarios could be processed either with the Acquisition Method set or a Specified Method Set, provided that the Method Set is set up as per the figures.

How to process some Injections with one Processing Method and other Injections with a different Processing Method?

STEP 9
We could create a Sample Set Method with two Method Sets, each with its own Processing Method. In this way we could process with the Acquisition Method Set, and Empower would follow the instructions from the Sample Set Method (Figure 9).

The last scenario is working with 3D detectors, such as photodiode array, fluorescence, and/or mass detectors like the QDa. This could lead to a variety of combinations of multiple channels of data that need to be processed.

STEP 10
In our final example, we collect 2D and 3D data from a PDA and an ACQUITY QDa mass detector (Figure 10).

STEP 11
In this case, we process with a Method set with the specific channels to be processed and their associated Processing Methods (Figure 11).

ADDITIONAL INFORMATION

This can be done with either the Pro or QuickStart interface.

The subject of validating chromatographic methods with the Empower Method Validation Manager will be revisited in Tip #331.