How to create a combined chromatogram out of two chromatograms from separate injections in Empower? - WKB92440

OBJECTIVE or GOAL

Create a combined chromatogram from two chromatograms originating from separate injections (samples) in Empower.

ENVIRONMENT

• Empower 3

PROCEDURE

1. Select the sample set that contains the two injections and right-click to select "alter sample".

2. Add the label "REF", for example, in the "Label" column for one of the two injections.

   1. E.g., Standard 1 contains Peak 1 in Channel B
   2. E.g., Standard 2 contains Peak 2 in Channel A
       
      

3. Create a derived channel in your method set.
   
    

4. In the first channel, select the channel of the first chromatogram you want to combine. In this example, it is channel A from Standard 2 containing Peak 2.

    

    

   

    

5. In the second channel, select "+" (Operator) 1 (Constant) x the second chromatogram channel you want to combine. In this example, it is channel B from Standard 1 containing Peak 1. Select the "From injection labeled" box and refer to the label of the injection containing the second chromatogram. In this example, we label the injection "REF".
    

6. Process the sample set with this method set to generate a chromatogram that is the combination of the two chromatograms. In this example, Channel A + Channel B containing Peak 1 and Peak 2. This enables you to perform calculations in one 2D channel with Peak 1 and Peak 2.

ADDITIONAL INFORMATION