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How Empower calculates signal-to-noise using the noise from within the same chromatogram - WKB65444

Article number: 65444

OBJECTIVE or GOAL

Calculating signal-to-noise using the noise from within the same chromatogram

ENVIRONMENT

  • Empower 2
  • Empower 3

PROCEDURE

This procedure requires a license for the system suitability option , and the project in which the data is being processed has the system suitability option enabled.

  1. Use the wizard to create a processing method, click on the image in red frame above to enter the Noise and Drift interface, as shown in the figure. Fill in the appropriate baseline noise start and end time according to the chromatogram, and the minimum slice width is 10 seconds, the maximum value is less than or equal to the difference between the start and end time of the baseline, and the upper limit is 300 seconds.
    clipboard_e008dc06355571bbdad049ff0c852865c.png

  2. Select the Suitability tab, check Calculate Suitability Results, enter 1 or the measured value in Void Volume Time. Select Peak to Peak Noise or Average Peak to Peak Noise for the S/N noise value, then click on File > Save > Processing Method in the Menu bar.
    clipboard_e28e9755f030d3f968925c716a29ecdb6.png

  3. After saving the processing method, click on Integrate clipboard_e1c3d8a6dc6e35df71caf9a83abe6f4fa.png, then Quantitate clipboard_ed067883aa7677c54f30fd8bb2f82f816.pngor Calibrate clipboard_edf9659d242b3f22f310b9dc6594ba5d0.png, you can use this processing method to calculate s/n.

  4. s/n is not displayed by default, you need to click clipboard_ef3b6c70473161b0f7c97eeadd4dc2d08.png go back to the Home page. Right click on Table Properties in the table . Uncheck the s/n, click Apply, then the s/n will be displayed in the form.
    clipboard_e3749e080155b6595470dc591a0ff9a40.png
    clipboard_e793edc2186fb053219cfd3291e671065.png

  5. If you want to display it in future, click Save Preferences under File.

ADDITIONAL INFORMATION

Tip #28: Calculating signal-to-noise ratio

id65444, baseline ripple, EMP2LIC, EMP2OPT, EMP2SW, EMP3GC, EMP3LIC, EMP3OPT, EMP3SW, EMPGC, EMPGPC, EMPLIC, EMPOWER2, EMPOWER3, EMPSW, SUP

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