- 2489 UV/Vis Detector
- 2998 Photodiode Array Detector
- ACQUITY UPLC PDA Detector
- ACQUITY UPLC Tunable UV Detector
No, you cannot. Some of the reasons are as follows:
1. The 2998 and the 2489 use different detection measurement.
The 2998 uses the formula:
Absorbance = -log [( Sn- Dn)/( Rn- Dn)]
S = signal obtained during sample analysis
D = signal obtained during dark current measurement
R = signal obtained from reference spectrum
n = diode number
The 2489 uses direct absorbance measurement (without any subtraction) by default.
2. The bandwidth on a 2489 (e.g., 5 nm) is different than the spectral resolution on a 2998 (the closest you can assign is 4.8 nm).
3. The detectors' flow cell and light alignment are different. The 2489 uses taper-slit, and the 2998 uses taper beam.
4. There is also a difference in the proximity of the flow cell to the light source. The lamp is much closer to the flow cell In a 2998.
Because of these differences and others, you cannot directly compare the data from a 2489 with that from a 2998.