What is the recommended flow rate for loading a sample on an SPE cartridge? - WKB66731
Article number: 66731
ENVIRONMENT
- SPE cartridges
- flow rate
- load step
- Oasis
- Sep-Pak
ANSWER
The following flow rates are based on the cartridge type or format:
Sep-Pak Classic, Plus, Oasis Plus formats: 1 mL/min
1cc Vac: 1 mL/min
3cc Vac: 1 mL/min
6cc Vac: 5 mL/min
12cc Vac: 10 mL/min
20cc Vac: 10-20 mL/min
35cc Vac: 20-50 mL/min
ADDITIONAL INFORMATION
Flow rate is a compromise among speed through the device, contact time with the sorbent bed, and volatility of the analyte of interest. The intent is to maximize sample recovery in the eluted fraction of interest.
For additional information, see the Sep-Pak Cartridges and Plates Care and Use Manual and Oasis Cartridges and 96-Well Plates.
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