Skip to main content
Waters

What is the loading capacity of a Sep-Pak C18 device? - WKB56342

Article number: 56342

ENVIRONMENT

  • Sep-Pak C18
  • tC18
  • loading capacity
  • mass load

ANSWER

The loading capacity of a Sep-Pak C18 is sample- and matrix-dependent. The approximate range is 1-10% of the total SPE device bed weight in the device. If the sample is a small molecule and water soluble, the load can go higher. If the sample is a peptide and more hydrophobic, the loading might be closer to 1% or less.

ADDITIONAL INFORMATION

For additional information, see the following:  SPE Primer and Beginner’s Guide to SPE (Solid Phase Extraction)

Not able to find a solution? Click here to request help.