- Sep-Pak C18
The loading capacity of a Sep-Pak C18 is sample- and matrix-dependent. The approximate range is 1-10% of the total SPE device bed weight in the device. If the sample is a small molecule and water soluble, the load can go higher. If the sample is a peptide and more hydrophobic, the loading might be closer to 1% or less.
For additional information, see the foillowing:
Beginner’s Guide to SPE (Solid Phase Extraction): http://www.waters.com/waters/partDetail.htm?partNumber=715003405