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What is the loading capacity of a Sep-Pak C18 device? - WKB56342

Article number: 56342

ENVIRONMENT

  • Sep-Pak C18

ANSWER

The loading capacity of a Sep-Pak C18 is sample- and matrix-dependent. The approximate range is 1-10% of the total SPE device bed weight in the device. If the sample is a small molecule and water soluble, the load can go higher. If the sample is a peptide and more hydrophobic, the loading might be closer to 1% or less.

ADDITIONAL INFORMATION

For additional information, see the foillowing:

SPE Primer: http://www.waters.com/waters/nav.htm?cid=10083488

Beginner’s Guide to SPE (Solid Phase Extraction): http://www.waters.com/waters/partDetail.htm?partNumber=715003405

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