What is the purpose of adding 4% phosphoric acid to serum or plasma prior to loading onto a SPE device? - WKB257794
Article number: 257794
ENVIRONMENT
- Solid-phase extraction (SPE)
- Sample pretreatment
- 4% phosphoric acid
ANSWER
4% phosphoric acid added 1:1 to serum or plasma is used to break any drug-to-protein binding or peptide-to-protein binding in the bio matrix prior to loading on an SPE device.
ADDITIONAL INFORMATION
The protein disruption enhances the recovery of the analyte of interest, when present in a bio matrix like serum or plasma.
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