What is the loading capacity for peptides on Sep-Pak C18 and tC18? - WKB24534

ENVIRONMENT

• WAT036820
• Peptides
• Sep-Pak C18
• Sep-Pak tC18
• loading capacity

ANSWER

The loading capacity for peptides on Sep-Pak C18 and tC18 is about 0.1% to 0.3% multiplied by the sorbent weight.

This loading capacity is estimated for peptides dissolved in a neat solution with no other matrix components present.

ADDITIONAL INFORMATION

For example, on part number WAT036820 - SEP-PAK TC18 Cart 1CC, 100mg cartridges, calculate estimated loading capacity as follows:

100 mg bed mass X 0.001 = 0.1 mg (100 µg)

100 mg bed mass X 0.003 = 0.3 mg (300 µg)

Note: Additional loading capacity may be observed due to the wide variety of peptide characteristics. A loading capacity study (breakthrough study) should be performed in order to verify true mass load. Loading capacity will be less if other hydrophobic matrix components are present in samples.

SPE Primer

Beginner’s Guide to SPE (Solid Phase Extraction)