How can I remove salt from a glucose sample prior to injecting samples onto a Sugar-Pak 1 column (WAT085188)? - WKB244133

ENVIRONMENT

• Solid-phase extraction (SPE)
• Sample preparation
• Salt removal
• Sugar-Pak 1
• Sep-Pak NH2 
• Sep-Pak PSA
• Sep-Pak Alumina A Classic

ANSWER

Use Sep-Pak NH2 or Sep-Pak PSA cartridges to capture and then elute sugars, allowing some of the salt to be washed away separately.

An alternative evaluation should be done using the Sep-Pak Alumina A, which is suitable for small molecular weight sugars from which acids like acetate need to be removed.

Experiment to find the sorbent that best cleans up the salt and provides the best results for the particular sample.

ADDITIONAL INFORMATION

Recommended starting point for Sep-Pak NH2 or PSA:

1. Condition the cartridge with 80:20 acetonitrile/H2O.

2. Dilute sample with acetonitrile so that the final concentration is 80:20 acetonitrile/H2O.

3. Pass sample through the cartridge. Sugars will retain and some salt will pass through, although some may retain.

4. Pass through a wash of 80:20 acetonitrile/H2O to remove residual salt in the interstitial spaces of the cartridge.

5. Elute the sugar in 50:50 acetonitrile/H2O to elute the sugar. 

• Elute with additional fractions of higher percentage water to find the best yield of sugar vs any residual salt that may still be present.

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Recommended starting point for Sep-Pak Alumina A Classic format:

1. Pass 5 mL of pure water through the Classic cartridge to pre-wet the Alumina A packing.

2. Place 10 mL (maximum) of sample in the syringe and slowly pass the sample through the cartridge.

a. Reject the first 5 mL.

b. Collect the next few milliliters of sample for injection.