What is the lysis/denaturation buffer referred to in the application note "Improved Oligonucleotide SPE-LC-MS Analysis Using MaxPeak High Performance technology" Lit code 720007019? - WKB234147
Article number: 234147
ENVIRONMENT
- Solid-phase extraction (SPE)
- Oligonucleotide analysis
- Lysis buffer
- Denaturation buffer
- Homogenization buffer
ANSWER
ADDITIONAL INFORMATION
Note that the application note highlights performance of the protocol in neat solution using the homogenizing/lysis reagents. If verifying a protocol from plasma or tissue, another sample pretreatment (such as LLE-SPE and/or Proteinase K digestion) may be required to effectively disrupt protein binding of the oligonucleotides from plasma proteins, to get good recovery. The pretreatment protocol will vary with different oligos and oligo sizes.
For additional information, see Improved Oligonucleotide SPE-LC-MS Analysis Using MaxPeak High Performance technology
and What is a suggested buffer to pre-treat urine to extract oligonucleotides using Oasis WAX sorbent?
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