How do I perform peptide desalting on Sep-Pak tC18 cartridges? - WKB190637
Article number: 190637
ENVIRONMENT
- Solid-phase extraction (SPE)
- Peptide desalting
- Sep-Pak tC18
- Oasis HLB
ANSWER
Use a protocol similar to the Oasis HLB Desalting of Peptide Mixture Prior to ESI-MS Analysis.
ADDITIONAL INFORMATION
Cartridge size, volumes, and conditions will need to be adjusted based on the peptide properties, complexity of sample matrix, and other factors.
Also see: What is the loading capacity for peptides on Sep-Pak C18 and tC18?
Waters Sep-Pak Sample Extraction Products Brochure
id190637, Electrospray