If I elute a DNPH cartridge with 3 mL instead of 10 mL, will my analyte concentrations be different? - WKB108387
Article number: 108387
ENVIRONMENT
- Sep-Pak
- Solid Phase Extraction (SPE)
- Air Quality Monitoring
ANSWER
Yes.
ADDITIONAL INFORMATION
If all DNPH-derivatized analytes are fully eluted in 3mL and also in 10mL, the concentration of DNPH-derivatized analytes in the 3mL will be 3.33x more concentrated compared to the concentration in the 10mL elution (prior to any dry-down steps).
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