What is the chromatographic impact of using a mobile phase outside the buffering range of a buffer? - WKB73772
Article number: 73772
ENVIRONMENT
- Chromatographic method developmentBuffer
- Mobile phase
- Buffer capacity
ANSWER
If the mobile phase is made up with buffer outside its buffering range, the chromatographic method will be less robust. The buffer will not be able to buffer changes in pH to the mobile phase as it sits in the container. Additional mobile phase controls may be needed depending on the nature of the analytes being separated.
ADDITIONAL INFORMATION
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