What volatile buffer is recommended for use with BEH SEC and ESI mass spec? - WKB73713
Article number: 73713
ENVIRONMENT
- Buffer
- Mobile phase
- Column
- BEH SEC
- UPLC
- ESI MS
- Mass spectrometer
- Electrospray ionization
ANSWER
Mobile phase containing 50 mM ammonium acetate has been used as an SEC buffer.
ADDITIONAL INFORMATION
For additional information see Analytical Scale Native SEC-MS for Antibody-Drug Conjugates (ADCs) Characterization and Analysis of Proteins by Size-Exclusion Chromatography Coupled with Mass Spectrometry Under Non-Denaturing Conditions.
See also What would be an SEC MS compatible mobile phase suggestion to use with a NIST mAb protein?
id73713, eluent, monoclonal antibody